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KMID : 0615720150320030041
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Jouranl of Korean Acupuncture and Moxibustion society
2015 Volume.32 No. 3 p.41 ~ p.51
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The Effects of LR3 and SP6 Acupuncture on Renal Damage in Streptozotocin-induced Diabetic Mice
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Lee Cho-In
Lee Hyun-Jong
Lee Yun-Kyu
Lim Seong-Chul
Kim Jae-Soo
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Abstract
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Objectives: This study was performed to investigate the effects of LR3 and SP6 acupuncture on renal damage in streptozotocin(STZ)-induced diabetic mice.
Methods: ICR male mice were stabilized for a week and divided into four groups: a normal mice group(N), no-acupuncture diabetic mice group(Control), LR3 acupuncture diabetic mice group(LR3), and SP6 acupuncture diabetic mice group(SP6). Diabetes was experimentally induced by intraperitoneal injection of STZ(150 mg/kg) in citrate buffer(pH 4.5). For two weeks, LR3 and SP6 acupunctures were administered bilaterally at each point once a day. After two weeks, the animals’ weight was measured and they underwent a laparotomy. Serum glucose and blood urea nitrogen(BUN) were measured from the blood taken from the heart. We measured glucose, reactive oxygen species(ROS), peroxynitrite(ONOO?) and thiobarbituric acid reactive substances(TBARS) in the kidney and compared expression levels of superoxide dismutases(SOD), glutathione peroxidase(GPx), nuclear factor-kappa B(NF-kB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase(iNOS) and Interleukin-1 beta(IL-1β).
Results: BUN significantly decreased in LR3, SP6 compared to the control group. LR3 showed significantly decreased glucose compared to the control group. LR3, SP6 significantly decreased in ROS and ONOO? compared to the control group. LR3 significantly decreased in TBARS compared to the control group. SP6 significantly increased in expressions of SOD-1, catalase, and GPx compared to the control group. LR3, SP6 significantly decreased in COX-2 compared to the control group. SP6 significantly decreased in IL-1β compared to the control group.
Conclusions: This study suggests that LR3 acupuncture may be effective in controlling glucose and lipid peroxidation and that SP6 acupuncture may have anti-oxidative and anti-inflammatory effects on renal damage in STZ-induced diabetic mice.
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KEYWORD
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Streptozotocin, Renal damage, Acupuncture, LR3, SP6
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